Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells

DNA extraction is usually the first step to perform molecular studies. This process can be nonviable due genomic (gDNA) commercial kits prices. Furthermore, available protocols generally have high specificity, limiting their use specific sources of biological material. In order reduce costs, optimize time and laboratory logistics, besides demonstrate a versatile protocol, present study worked on an efficient protocol from somatic non-somatic cells, using material sheep as model. For that, gDNA was extracted whole blood, spermatozoa, hair bulb collected three adult sheep, transported at 5ºC stored -20ºC until lab procedures. After extraction, concentration purity were evaluated in nano spectrophotometer. blood greater (p < 0.05) than which turn superior spermatozoa. Also, and, followed by, sperm showed when compared cells. Adapting originally developed for bovine enabled obtain isolate different nucleated cells